this method of calculating the diabetic panel tends to be precise due to independence of factors associated with nutrition and renal reabsorption
1.Sample collection from the patient's blood.
2.Preparation of the sample by separating the hemoglobin from other components using a centrifuge or other methods.
3.Treatment of the separated hemoglobin with specific reagents such as sodium borohydride to convert the glycated hemoglobin to an unglycated form.
4.The converted unglycated hemoglobin is then separated from the remaining sample components using chromatography, electrophoresis, or other separation methods.
5.The concentration of HbA1c in the sample is then determined using a spectrophotometer.
6.The spectrophotometer measures the absorbance of the sample at a specific wavelength and compares it to a standard calibration curve to determine the HbA1c concentration, there are three tubes, one of which is for device zeroing containing distilled water, the second, is for the standard, we add the standard from the kit in amounts that vary according to the kit plus the reagent, the third is for the sample, same steps for the standard tube but insteand of the standard we add the blood sample.
7.The HbA1c concentration is reported as a percentage of total hemoglobin or in mmol/mol. The equation is:
HbA1c concentration (mmol/mol) = (HbA1c concentration % – 2.15) x 10.929.
7.1that equation is a general one, the one we did was Asample/Astandard×concentration of the Standard
here's a picture that may be benificial for HBA1C test diagnosis:
that was simply it, dont get diabetes.. it sucks
